The Action of Two Sulfhydryl Compounds on Normal Human Red Cells. Relationship to Red Cells of Paroxysmal Nocturnal Hemoglobinuria.
نویسندگان
چکیده
characteristic lesion of the erythrocytes in the laboratory. Attempts along this line were made by Rodbard1 who, assuming that the erythrocytic defect was localized in the lipid fraction of the stroma, succeeded on a few occasions in obtaining lysis of normal red cells in acidified serum-which occurs also under the same conditions to PNH red cells2-by means of repeated washing with dilute alcoholic solutions. However, the results were not constant and reproducible. By contrast, the results obtained by Metz et al.,3 using an acetylcholinesterase inhibitor (OMPA), and by Ghiotto et al. (quoted by De Sandre et al.4) using another acetylcholinesterase inhibitor (physostigmine), were complete failures. So far, the best results have been obtained by Yachnin et al.,5 who have reported that, when normal red cells are altered by means of various proteolytic enzymes (trypsin, ficin, bromelin and papain), cholera vibrio filtrate, influenza virus or sodium periodate, it is possible to obtain erythrocytes whose in vitro behaviour is similar to that of PNH red cells, although differing from the latter in some respects. The purpose of this report is to describe the results obtained by treating normal human red cells with AET (2-amino-ethylisothiouronium bromide) and with cysteine; both substances contain SH groups, belong to the group of sulfhydryl compounds, but are not enzymes in nature.
منابع مشابه
Normal Human Lymphocytes Treated in Vitro With the Sulfhydryl Compound AET: Relationship to the Lymphocytes of Paroxysmal Nocturnal Hemoglobinuria
Because in vitro treatment with the sulfhydrsl compound AET is known to alter normal red cells in such a way that they become highly susceptible to the lytic action of complement, an investigation was carried out to evaluate whether the same substance could modify normal lymphocytes in a similar way. The lymphocytes from paroxysmal nocturnal hemoglobinuria were also studied as a comparison. AET...
متن کاملNormal Human Lymphocytes Treated in Vitro With the Sulfhydryl Compound AET: Relationship to the Lymphocytes of Paroxysmal Nocturnal Hemoglobinuria
Because in vitro treatment with the sulfhydrsl compound AET is known to alter normal red cells in such a way that they become highly susceptible to the lytic action of complement, an investigation was carried out to evaluate whether the same substance could modify normal lymphocytes in a similar way. The lymphocytes from paroxysmal nocturnal hemoglobinuria were also studied as a comparison. AET...
متن کاملNormal human lymphocytes treated in vitro with the sulfhydryl compound AET: relationship to the lymphocytes of paroxysmal nocturnal hemoglobinuria.
Because in vitro treatment with the sulfhydrsl compound AET is known to alter normal red cells in such a way that they become highly susceptible to the lytic action of complement, an investigation was carried out to evaluate whether the same substance could modify normal lymphocytes in a similar way. The lymphocytes from paroxysmal nocturnal hemoglobinuria were also studied as a comparison. AET...
متن کاملImmune lysis of normal human and paroxysmal nocturnal hemoglobinuria (PNH) red blood cells. I. The sensitivity of PNH red cells to lysis by complement and specific antibody.
متن کامل
The control of complement activation by the blood cells in paroxysmal nocturnal hemoglobinuria.
By Wendell F. Rosse S INCE THE CLASSIC studies of Ham and Dingle,’ it has been known that the red blood cells of patients with paroxysmal nocturnal hemoglobinuria (PNH) are unusually susceptible to the hemolytic action of complement. There are two major types of abnormal cells in this syndrome: the PNH II red blood cells are moderately sensitive to the lytic action of complement (three to six t...
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عنوان ژورنال:
- Blood
دوره 25 شماره
صفحات -
تاریخ انتشار 1965